Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.

摘要: It has previously been shown that the acetylated forms of histone H4 are depleted or absent in both constitutive, centric heterochromatin and facultative inactive X chromosome (Xi) female cells. By immunostaining metaphase chromosomes from human lymphocytes with antibodies to isoforms histones H2A H3, we now show these too underacetylated Xi heterochromatin. shows two prominent regions residual H3 acetylation, one encompassing pseudoautosomal region at end short arm about Xg22. Both have be sites acetylation. acetylation on is higher overall than particularly high around region, but not The results suggest least some effects chromosomal structure function shared by H2A.