The influence of Matrigel or growth factor reduced Matrigel on human intervertebral disc cell growth and proliferation.

摘要: MatrigelTM (reconstituted basement membrane extract) is a potent inducer of cell growth and differentiation in vitro. This study examined phenotypic variation proliferative responses human annular intervertebral disc cells vitro Growth Factor Reduced (GFR-MatrigelTM). Cells from age- gender-matched control subjects patients with degenerative disease were grown either on the surface of, or suspended within, matrices. Disc grew well top both matrices spontaneously forming projections. within matrix migrated gel to form colonies. Increased colony formation was seen young patient (p<0.05). Old showed increased proliferation GFR-MatrigelTM compared MatrigelTMW. hen surface, donor GFR-MatrigelTMc ompared MatrigelTM. Cellular significantly greater inside 3-dimensional environment than twodimensional monolayer environment. had when GFRMatrigelTMc MatrigelTMT. hese studies serve as baseline for subsequent investigations regarding effects cytokines increase our knowledge influence extracellular proliferation.