Dissociative and Nondissociative Models for Culture of Human Eccrine Glands for Toxicology Testing and Tissue Engineering Applications
作者： Kelsea M. Hubka , Danielle Wu , Daniel A. Harrington , Jessica C. Langer , Thomas Pocard
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摘要: Abstract Current in vitro models of human skin lack appendages, which are an integral component native and necessary structures reconstructed for applications such as testing pharmacological agents, basic discovery, tissue engineering. We describe our development three complementary eccrine gland culture models: (1) nondissociative model whole glands attached to epidermis, (2) detached from (3) dissociative cells. In each model, were encapsulated cultured modifiable hyaluronic acid-based hydrogels. Glands these cultures stained positive ductal secretory coil-specific markers, demonstrating the ability continue express appropriate phenotypic markers. The maintain full, intact ideal discovery toxicology testin...
sci-hub.se 参考文章(36)
Xia Lei, Bo Liu, Jinjin Wu, Yuangang Lu, Yadong Yang, Matrigel-induced tubular morphogenesis of human eccrine sweat gland epithelial cells. Anatomical Record-advances in Integrative Anatomy and Evolutionary Biology. ,vol. 294, pp. 1525- 1531 ,(2011) , 10.1002/AR.21459
Thomas Biedermann, Luca Pontiggia, Sophie Böttcher-Haberzeth, Sasha Tharakan, Erik Braziulis, Clemens Schiestl, Martin Meuli, Ernst Reichmann, Human Eccrine Sweat Gland Cells Can Reconstitute a Stratified Epidermis Journal of Investigative Dermatology. ,vol. 130, pp. 1996- 2009 ,(2010) , 10.1038/JID.2010.83
Walter K. F. Seelentag, Ursula Günthert, Parvin Saremaslani, Eva Futo, Madeleine Pfaltz, Philipp U. Heitz, Jürgen Roth, CD44 STANDARD AND VARIANT ISOFORM EXPRESSION IN NORMAL HUMAN SKIN APPENDAGES AND EPIDERMIS Histochemistry and Cell Biology. ,vol. 106, pp. 283- 289 ,(1996) , 10.1007/BF02473238
Sabine Nagel, Franziska Rohr, Caroline Weber, Janina Kier, Frank Siemers, Charli Kruse, Sandra Danner, Matthias Brandenburger, Anna Emilia Matthiessen, Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and Can Be Propagated Robustly In Vitro PLoS ONE. ,vol. 8, pp. e78365- ,(2013) , 10.1371/JOURNAL.PONE.0078365
Hjalmar Kurzen, Hans Berger, Claudia Jäger, Wolfgang Hartschuh, Helmut Näher, Alexei Gratchev, Sergij Goerdt, Martin Deichmann, Phenotypical and Molecular Profiling of the Extraneuronal Cholinergic System of the Skin Journal of Investigative Dermatology. ,vol. 123, pp. 937- 949 ,(2004) , 10.1111/J.0022-202X.2004.23425.X
Xiao Zheng Shu, Yanchun Liu, Fabio Palumbo, Glenn D Prestwich, Disulfide-crosslinked hyaluronan-gelatin hydrogel films: a covalent mimic of the extracellular matrix for in vitro cell growth. Biomaterials. ,vol. 24, pp. 3825- 3834 ,(2003) , 10.1016/S0142-9612(03)00267-9
Haihong Li, Lu Chen, Mingjun Zhang, Shijie Tang, Xiaobing Fu, Three-dimensional culture and identification of human eccrine sweat glands in matrigel basement membrane matrix. Cell and Tissue Research. ,vol. 354, pp. 897- 902 ,(2013) , 10.1007/S00441-013-1718-3
Xia Lei, Jinjin Wu, Yuangang Lu, Tangyou Zhu, Effects of acetylcholine chloride on intracellular calcium concentration of cultured sweat gland epithelial cells Archives of Dermatological Research. ,vol. 300, pp. 335- 341 ,(2008) , 10.1007/S00403-008-0847-0
Kevin T. Dicker, Lisa A. Gurski, Swati Pradhan-Bhatt, Robert L. Witt, Mary C. Farach-Carson, Xinqiao Jia, Hyaluronan: A simple polysaccharide with diverse biological functions☆ Acta Biomaterialia. ,vol. 10, pp. 1558- 1570 ,(2014) , 10.1016/J.ACTBIO.2013.12.019
P.A. Botham, D.A. Basketter, T. Maurer, D. Mueller, M. Potokar, W.J. Bontinck, Skin sensitization--a critical review of predictive test methods in animals and man. Food and Chemical Toxicology. ,vol. 29, pp. 275- 286 ,(1991) , 10.1016/0278-6915(91)90025-3