Recovery and composition of microparticles after snap-freezing depends on thawing temperature
作者： Arne Trummer , Christiane De Rop , Andreas Tiede , Arnold Ganser , Roswith Eisert
DOI: 10.1097/MBC.0B013E32831BE9C5
关键词:
摘要: Consent regarding the correct processing and storage of blood microparticles is lacking different protocols for freeze-thaw cycle exist. Therefore, three thawing procedures were evaluated their influence on recovery composition microparticles. Microparticles prepared by TRAP-6 or A23187 stimulation platelet-rich plasma from smokers nonsmokers (n = 8), an endothelial cell line directly obtained platelet-free septic patients 5). After snap-freezing in liquid nitrogen samples thawed at 37 degrees, room temperature ice staining was carried out with Annexin V-Cy5 as well fluorescein isothiocyanate (FITC) phycoerythrin (PE) labelled antibodies isotype controls. Microparticle concentrations determined means Trucount tubes. Recovery platelet significantly reduced when (P 0.001 all antigens) compared two other techniques 0.6 degrees P 0.7 temperature, respectively) whereas origin appeared to be less influenced. There a strong trend towards altered microparticle counts detected CD41 showed stronger decrease than V enumeration 0.07). For detection snap-frozen, should C water bath but not ice.
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