Chromosomal Damage Induced by Herbicide Contamination at Concentrations Observed in Public Water Supplies

摘要: Herbicide contamination of natural resources, specifically groundwater, has drawn considerable public attention in recent years. Although deleterious effects herbicides at large dosages have been well documented, information is lacking about the possible herbicide levels that are found water supplies. Chromosomal damage examined by flow cytometry on Chinese hamster ovary (CHO) cells exposed to three (atrazine, simazine, and bentazon) concentrations deemed safe U.S. Environmental Protection Agency (USEPA) for drinking water. A known clastogen (ara-C) also included as a reference comparing magnitude chromosomal caused herbicides. was assessed measuring coefficient variation (CV) percent chromosomes present larger chromosome distribution peaks. Exposure atrazine (6-chloro-N-ethyl-N'-(1-metbylethyl)-1,3,5-triazine-2,4-diamine) increased CV largest peak considered USEPA. Chromosomes both simazine (6-chloro-N,N'-dietbyl-1,3,5-triazine-2,4-diamine) bentazon (3-(1-methylethyl)-(1H)-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide) did not exhibit damage. Further analyses carried out equal supplies revealed potential induce breakages CHO cells. Atrazine were manyfold greater than limits exhibited true clastogenic nature like ara-C. The results provide evidence further investigations health risk consuming contaminated with atrazine.