Quantitative molecular characterization of bovine vitreous and lens with non-invasive dynamic light scattering.

摘要: The non-invasive technique of dynamic light scattering (DLS) was used to quantitatively characterize vitreous and lens structure on a molecular level by measuring the sizes predominant particles mapping three-dimensional topographic distribution these structural macromolecules in three spatial dimensions. results DLS measurements five fresh adult bovine eyes were compared model solutions hyaluronan (HA) collagen (Coll). In obtained from excised samples gel liquid solutions. Measurements whole at multiple points posterior generate 'map' structure. macromolecule similarly characterized.In each (Bo Vit) specimen, predominantly detected two distinct particles, which differed diffusion properties hence size. Comparisons with demonstrated that most likely corresponded Coll HA components vitreous. Three-dimensional Bo Vit found heterogeneity throughout body, different particle size distributions for loci. contrast, more homogeneous. Thus, can quantitate average map their distribution. This method assess macromolecular should be useful clinical as well experimental applications health disease.