The state of ferriprotoporphyrin IX in malaria pigment.

摘要: To evaluate the state of ferriprotoporphyrin IX (FP) in malaria pigment, mouse erythrocytes infected with Plasmodium berghei NYU-2 parasites were lysed by hypotonic shock, and hemoglobin other soluble material removed extensive washing. The amount FP recovered insoluble pellet was 2.1 mumol/ml packed erythrocytes, which approximately 1% attributable to contamination. This crude preparation then digested a nonspecific protease from Streptomyces griseus extracted chloroform/methanol. residue dark brown had spectral solubility properties characteristic various different preparations contained 82 99% weight. By elemental analysis, highly purified no chlorine an oxygen content consistent 1 mol hydroxyl/mol (oxygen content: calculated, 12.6%; found, 12.5%). In comparison hematin purchased Sigma, measured 14.7%, low form pigment remarkably less ethanol, 3% sodium bicarbonate, chloroform.