An hplc light-scattering detector for biopolymers

摘要: A high angle light scattering detector using classical Rayleigh scattering. intensity arc source, filtered to leave only one wavelength illuminates a flow cell. Through the cell, very small particles such as biological proteins in solution after separation by HPLC or some other means. UV generates data regarding weight concentration of eluting and scattered collecting at angles approximately 90° signal. The incident is also measured. average molecular then computed data, simplified mathematical relationship from which size factor P viral coefficients have been eliminated.