作者: Jyrki K. Mäkelä , Tuula Vuorio , Eero Vuorio
DOI: 10.1016/0167-4781(90)90037-3
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摘要: Abstract Five human skin fibroblast lines were studied for type I collagen production and procollagen mRNA levels through the different growth phases. The cells plated at low density followed 11 days daily intervals stages of rapid visual confluency until cultures reached stationary phase. Each day one culture flask was labeled with [ 3 H]proline 24 h, analyzed radiolabeled into medium. cell layers counted subjected to isolation cytoplasmic RNA determination levels. results revealed an approx. 2-fold increase in when confluency. Thereafter synthesis rates remained relatively constant, although a decreasing tendency both parameters observed upon further culturing. confirm that is important are used measurement or For proα2(I) 1193 bp cDNA clone constructed using extracted from fetal calvaria. Sequencing some nucleotide amino acid differences between previously published sequences. This suggests presence more individual variation coding sequences than expected.