Growth-dependent modulation of type I collagen production and mRNA levels in cultured human skin fibroblasts

作者: Jyrki K. Mäkelä , Tuula Vuorio , Eero Vuorio

DOI: 10.1016/0167-4781(90)90037-3

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摘要: Abstract Five human skin fibroblast lines were studied for type I collagen production and procollagen mRNA levels through the different growth phases. The cells plated at low density followed 11 days daily intervals stages of rapid visual confluency until cultures reached stationary phase. Each day one culture flask was labeled with [ 3 H]proline 24 h, analyzed radiolabeled into medium. cell layers counted subjected to isolation cytoplasmic RNA determination levels. results revealed an approx. 2-fold increase in when confluency. Thereafter synthesis rates remained relatively constant, although a decreasing tendency both parameters observed upon further culturing. confirm that is important are used measurement or For proα2(I) 1193 bp cDNA clone constructed using extracted from fetal calvaria. Sequencing some nucleotide amino acid differences between previously published sequences. This suggests presence more individual variation coding sequences than expected.

参考文章(36)
M Aumailley, T Krieg, G Razaka, P K Müller, H Bricaud, Influence of cell density on collagen biosynthesis in fibroblast cultures Biochemical Journal. ,vol. 206, pp. 505- 510 ,(1982) , 10.1042/BJ2060505
W de Wet, M Bernard, V Benson-Chanda, M L Chu, L Dickson, D Weil, F Ramirez, Organization of the human pro-alpha 2(I) collagen gene. Journal of Biological Chemistry. ,vol. 262, pp. 16032- 16036 ,(1987) , 10.1016/S0021-9258(18)47691-0
Francis S. Collins, Sherman M. Weissman, The molecular genetics of human hemoglobin. Progress in Nucleic Acid Research and Molecular Biology. ,vol. 31, pp. 315- 462 ,(1984) , 10.1016/S0079-6603(08)60382-7
P. Tolstoshev, R.A. Berg, S.I. Rennard, K.H. Bradley, B.C. Trapnell, R.G. Crystal, Procollagen production and procollagen messenger RNA levels and activity in human lung fibroblasts during periods of rapid and stationary growth. Journal of Biological Chemistry. ,vol. 256, pp. 3135- 3140 ,(1981) , 10.1016/S0021-9258(19)69735-8
B A White, F C Bancroft, Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples. Journal of Biological Chemistry. ,vol. 257, pp. 8569- 8572 ,(1982) , 10.1016/S0021-9258(18)34158-9
Richard Mayne, Robert E. Burgeson, Structure and function of collagen types Academic Press. ,(1987)