Immune responses and protection induced by Brucella suis S2 bacterial ghosts in mice
作者： Jun Liu , Yi Li , Yang Sun , Xue Ji , Lingwei Zhu
DOI: 10.1016/J.VETIMM.2015.04.008
关键词:
摘要: With the purpose of generating Brucella suis bacterial ghosts and investigating immunogenicity as a vaccine candidate, lysis gene E temperature-sensitive regulator cassette were cloned into shuttle plasmid, pBBR1MCS-2, for construction recombinant pBBR1MCS-E. pBBR1MCS-E was then introduced attenuated B. live S2 bacteria, resultant transformants used production (BSGs) by inducing expression. The BSGs characterized observing their morphology transmission electron microscopy. safety further evaluated using murine model, result suggested that BSG safe formalin-killed suis. In mice, demonstrated similar capacity pathogen-specific serum IgG antibody response, spleen CD3+ CD4+ T cell responses, induce secretion gamma interferon interleukin-4, protection levels against melitensis 16M challenge, vaccine. These data suggesting could confer infection in mouse model disease may be developed new candidate infection.
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