BH3 profiling discriminates the anti‑apoptotic status of 5‑fluorouracil‑resistant colon cancer cells

摘要: 5‑Fluorouracil (5‑FU) is a cytotoxic anticancer drug commonly used for patients with advanced colon cancer. This effectively reduces the size of tumors to certain degree; however, cancer cells can gradually acquire resistance, resulting in disease progression. To identify mechanism 5‑FU we established three 5‑FU‑resistant cell lines and analyzed both apoptosis‑related protein expression levels BH3 profiling. These acquired apoptotic resistance 5‑FU. Although were altered each line variably, profiling indicated BCLXL dependence HT‑29 only. Functional inhibition not only sensitized apoptosis but also overcame resistance. The BIM was preferentially sequestered, thereby on survival. Additionally, vivo models showed that controlled tumor results indicate facilitates identification functional role anti‑apoptotic proteins during has clinical implications targeting specific such as BCLXL.