Minichromosome maintenance protein 7 regulates phagocytosis in kuruma shrimp Marsupenaeus japonicas against white spot syndrome virus

摘要: Minichromosome maintenance protein (MCM7) belongs to the MCM family and participates in complex by playing a role cell replication cycle chromosome initiation of eukaryotes. Previously, we found that several genes, including MCM7, were over-expressed Drosophila melanogaster after white spot syndrome virus (WSSV) infection. In this study, aimed further research MCM7 kuruma shrimp, Marsupenaeus japonicus (mjMCM7) determine its innate immune system. To end, cloned entire 2307-bp mjMCM7 sequence, 1974-bp open reading frame (ORF) encoding 658-aa-long protein. Real-time PCR showed gene was primarily expressed hemolymph hepatopancreas shrimp challenged with WSSV. Gene function study carried out knocking down expression using small interference RNA (siRNA). The results revealed β-actin, hemocyanin, prophenoloxidase (proPO) tumor necrosis factor-α (TNF-α) up-regulated while cytoskeleton proteins such as myosin Rho significantly down-regulated at 24 h treatment. indicate possible relationship between system, suggest may play phagocytosis. After WSSV challenge, copies mortality count both higher MCM7-siRNA-treated groups 60 h treatment, approached control over time. phagocytosis rate lower group than group. findings confirm positively regulates plays an important against These could help researchers understand reveal potential immunity invertebrates.