Gene Expression Profiling of MCF10A Breast Epithelial Cells Exposed to IOERT.

摘要: Background/Aim: Intraoperative electron radiation therapy (IOERT) is a therapeutic approach that delivers single high dose of ionizing (IR) directly to the tumor bed during cancer surgery. The main goal IOERT counteract growth by acting on residual cells as well preserve healthy surrounding tissue from side-effects therapy. radiobiology response IR topic interest which may contribute avoiding impairment normal and organ function reducing risks secondary cancer. purpose study was highlight cell gene expression responses following treatment in human non- tumorigenic MCF10A line order find new potential biomarkers radiosensitivity/radioresistance. Material Methods: Gene-expression profiling treated with 9 23 Gy doses (IOERT boost exclusive treatment, respectively), performed whole- genome cDNA microarrays. Real-time quantitative reverse transcription (qRT-PCR), immunofluorescence immunoblot experiments were carried out validate candidate biomarkers. Clonogenic tests morphological evaluations examine cellular effects induced also conducted. Results: revealed dose-dependent gene- profile specific key genes be proposed novel markers radiosensitivity. Our results show consistent differences non-tumorigenic tolerance molecular different IOERTs. In particular, after exposure, selection radioresistant fraction observed. Conclusion: possibility clarifying strategies adopted choosing between death or survival IR-induced damage opens-up avenues for proper personalized schedule. differs conventional radiotherapy (RT), since large employed surgery, either an 21-23 advanced 9-12 Gy. use breast (BC) has increased due development partial irradiation (PBI) strategy intent recurrence. This segmental RT replaces whole-breast based discovery approximately 85% local relapses are localized same quadrant primary removed (1-6). Preliminary PBI IOERT, both seem promising terms disease control, however few data have been collected long-term toxicity, stress mechanisms specifically high-