Comparative analysis of the CD19+ and CD138+ cell antibody repertoires in the cerebrospinal fluid of patients with multiple sclerosis.

摘要: Increased amounts of intrathecally synthesized IgG and oligoclonal bands have long been recognized as a hallmark multiple sclerosis (MS). B cells plasma are components the inflammatory infiltrates in both active chronic MS lesions, increased numbers these present cerebrospinal fluid (CSF). Single-cell RT-PCR was used to analyze CD19+ cell CD138+ populations CSF two patients with clinically definite one patient whose obtained after isolated syndrome, but before second episode. Sequence analysis amplified V region sequences identified rearranged germline segments, extent somatic mutation, clonal relationships within between three patients. Expanded clones were detected each all repertoire more restricted. However, little if any significant sequence overlap observed repertoires donor. Detection by single-cell PCR will facilitate vitro production recombinant Abs useful identifying disease-relevant Ags.