In vitro and in vivo models of cartilage injury.

摘要: Our previous study1 demonstrated that chondrocytes undergo apoptotis in response to mechanical injury full-thickness cartilage explants. To validate this response, several models of across species and vivo were examined. Full-thickness explants harvested from weight-bearing portions adult bovine femoral condyles, 5-mm-diameter disks punched out with a dermal punch. Explants allowed stabilize Dulbecco modified Eagle medium supplemented 10% fetal serum for forty-eight hours. then divided into two groups: load control. The group underwent single 500-msec 30% strain radially unconfined compression. control was not loaded. A found generate more consistent than the loading protocol1. At ninety-six hours after injury, histologic examination number apoptotic cells counted use TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling). Apoptosis confirmed selected samples by electron microscopy immunostaining neo-epitope cytokeratin. experiment repeated normal human articular condyles postmortem donors. Osteochondral model: determine whether presence subchondral bone affected whole rabbit patellae …