T lymphocyte migration to lymph nodes is maintained during homeostatic proliferation.

摘要: The immune system maintains appropriate cell numbers through regulation of proliferation and death. Normal tissue distribution lymphocytes is maintained expression specific adhesion molecules chemokine receptors such as L-selectin CCR7, respectively. Lymphocyte insufficiency or lymphopenia induces homeostatic existing to increase numbers. Interestingly, T a phenotypic change from naive- memory-type cell. Naive cells recirculate between blood lymphoid tissues whereas memory migrate nonlymphoid sites skin gut. To assess effects on migratory ability cells, murine model lymphopenia-induced was used. Carboxyfluorescein diacetate, succinimidyl ester-labeled wild-type splenocytes were adoptively transferred into recombination activation gene-1-deficient mice analyzed by flow cytometry, in vitro chemotactic vivo migration assays, immunofluorescence microscopy. Homeostatically proliferated acquired mixed CD44high L-selectinhigh CCR7low phenotype. Consistent with this, chemotaxis secondary reduced 22%-34%. By contrast, no differences found for entry lymph nodes during assays. Therefore, that have undergone using mechanisms similar naive cells.