Determination of anthocyanins in cranberry fruit and cranberry fruit products by high-performance liquid chromatography with ultraviolet detection: single-laboratory validation.

摘要: A single-laboratory validation study was conducted on an HPLC method for the detection and quantification of cyanidin-3-O-galactoside (C3Ga), cyanidin-3-O-glucoside (C3GI), cyanidin-3-O-arabinoside (C3Ar), peonidin-3-O-galactoside (P3Ga), peonidin-3-O-arabinoside (P3Ar) in cranberry fruit (Vaccinium macrocarpon Aiton) raw material finished products. An extraction procedure using a combination sonication shaking with acidified methanol optimized all five anthocyanins freeze-dried products (commercial extract powder, juice, juice cocktail). Final solutions were analyzed by C18 RP column. Calibration curves anthocyanin concentrations had correlation coefficients (r2) > or = 99.8%. The limits C3Ga, C3Gl, C3Ar, P3Ga, P3Ar estimated to be 0.018, 0.016, 0.006, 0.013, 0.011 microg/mL, respectively. Separation achieved chromatographic run time 35 min binary mobile phase gradient elution. Quantitative determination performed triplicate four test materials each 3 days (n 12) resulted RSD(r) from 1.77 3.31%. Analytical range, as defined calibration curves, 0.57-36.53 microg/mL 0.15-9.83 C3GI, 0.28-17.67 1.01-64.71 0.42-27.14 P3Ar. For solid prepared described method, this translates 0.06-3.65 mglg 0.02-0.98 mg/g 0.03-1.77 0.10-6.47 0.04-2.71