BrainFilm, a novel technique for physical compression of 3D brain slices for efficient image acquisition and post-processing

摘要: Tissue clearing enables us to observe thick tissue at a single cell resolution by reducing light scattering and refractive index matching. However, imaging of large volume for 3D reconstruction requires great deal time, cost, efforts. Few methods have been developed transcend these limitations mechanical compression or isotropic shrinkage. shrinkage significantly lessens the burden; however, there is an inevitable trade-off with image resolution. Here, we "BrainFilm" technique compress cleared Z-axis dehydration, without alteration XY-axis. The was approximately 90%, resulted in substantial reduction acquisition time data size. BrainFilm successfully used trace characterize morphology biocytin-labelled neurons following electrophysiological recording GFP-labelled long nerve projections irregular tissues such as limb mouse embryo. Thus, versatile tool that can be applied diverse studies which spatial information dispensable.