Chronic Exposure to a High-Fat Diet Induces Hepatic Steatosis, Impairs Nitric Oxide Bioavailability, and Modifies the Mitochondrial Proteome in Mice

摘要: Abstract Obesity-related pathologies, such as nonalcoholic fatty liver disease, are linked to mitochondrial dysfunction and nitric oxide (NO) deficiency. Herein, we tested the hypothesis that a high-fat diet (HFD) modifies proteome alters proteins involved in NO metabolism, namely arginase 1 endothelial synthase. Male C57BL/6 mice were fed control or HFD mitochondria isolated for proteomics reactive oxygen species measurements. Steatosis hepatocyte ballooning present livers of mice, with no pathology observed controls. had increased serum glucose decreased adiponectin. Mitochondrial was after 8 weeks but at 16 compared control, which accompanied by uncoupling protein 2. Using proteomics, 22 altered consequence HFD. This cohort consists oxidative phosphorylation, lipid sulfur amino acid ...