Microenvironment dependent gene expression signatures in reprogrammed human colon normal and cancer cell lines

摘要: Since the first evidence suggesting existence of stem-like cancer cells, process cells reprogramming to stem cell state remains as an attractive tool for stemness research. Current knowledge in field stemness, indicates that microenvironment is a fundamental regulator behavior. With regard this, we investigated changes genome wide gene expression reprogrammed human colon normal epithelial CRL-1831 and carcinoma DLD1 lines grown under more physiologically relevant three-dimensional (3D) culture compared 2D monolayer. Whole were evaluated both cultured 3D conditions over monolayer by microarray analysis. To evaluate biological significance changes, performed pathway enrichment analysis using Kyoto Encyclopedia Genes Genomes (KEGG) database. Gene network was used study relationships between differentially expressed genes (DEGs) functional categories GeneMANIA Cytoscape toolkit. In total, identified 3228 2654 lines, respectively. Furthermore, 1097 commonly regulated lines. KEGG revealed total 129 101 pathways iPSC-CRL-1831 CSC-DLD1, respectively, enriched. Next, grouped these into three categories: transformation/metastasis, interaction, stemness. β-catenin (CTNNB1) confirmed hub all categories. Our present findings suggest common epithelium (iPSC-CRL-1831) adenocarcinoma (CSC-DLD1) microenvironment. addition, demonstrated important transformation tumor metastatic activity are altered during transfer from conditions. Thus, indicate potential models enriched identification new therapeutic targets treatment.