T Cell-Specific Expression of the Human TNF-α Gene Involves a Functional and Highly Conserved Chromatin Signature in Intron 3

摘要: Using a phylogenetic approach, we identified highly conserved sequences within intron 3 of the human TNF-α gene. These form cell type-specific DNase I hypersensitivity sites and display DNA-protein contacts in vivo genomic footprints. Consistent with these results, confers specific activity upon reporter gene Jurkat T cells, but not THP-1 monocytic cells. Thus, using combinatorial approach analysis, footprinting, transfection demonstrate that intronic regulatory elements are involved regulation expression.