Transposable Phage Mu.

摘要: Transposable phage Mu has played a major role in elucidating the mechanism of movement mobile DNA elements. The high efficiency transposition facilitated detailed biochemical dissection reaction mechanism, as well protein and elements that regulate transpososome assembly function. deduced phosphotransfer involves in-line orientation metal ion-activated hydroxyl groups for nucleophilic attack on reactive diester bonds, appears to be used by all transposable examined date. A crystal structure is available. differs from other encoding unique adaptations promote its viral lifestyle. These include multiple (enhancer, SGS) (MuB, HU, IHF) enable efficient end synapsis, target capture, low specificity, immunity near or into itself, mechanisms recruiting host repair replication machineries resolve intermediates. MuB functions, including capture immunity. SGS element promotes gyrase-mediated enhancer, aided HU IHF, participates directing topological architecture synapse. function these important during both lysogenic lytic phases. Enhancer properties have been exploited design mini-Mu vectors genetic engineering. ends assembled active transpososomes delivered directly bacterial, yeast, human genomes, where they integrate efficiently, may prove useful gene therapy.