Complex Size and Surface Charge Determine Nucleic Acid Transfer by Fusogenic Liposomes

摘要: Highly efficient, biocompatible, and fast nucleic acid delivery methods are essential for biomedical applications research. At present, two main strategies used to this end. In non-viral transfection liposome- or polymer-based formulations transfer cargo into cells via endocytosis, whereas viral carriers enable direct the cell cytoplasm. Here, we introduce a new generation of liposomes delivery, which immediately fuse with cellular plasma membrane upon contact functional directly For maximum fusion efficiency combined high transfer, acids had be complexed partially neutralized before incorporation fusogenic liposomes. Among various neutralization agents tested, small, linear, positively charged polymers yielded best complex properties. Systematic variation liposomal composition complexation identified surface charge as well particle size parameters cargo-liposome interaction subsequent induction. Optimized protocols were tested efficient different kinds like plasmid DNA, messenger RNA, short-interfering RNA mammalian in culture primary tissues.