THE INFLUENCE OF TISSUE SPECIFIC CARDIAC AND SKELETAL MUSCLE EXTRACELLULAR MATRIX ON MUSCLE REMODELING

摘要: Volumetric muscle loss (VML) in skeletal and of cardiac function after myocardial infarction lack viable treatment options for functional tissue restoration. Previously investigated strategies such as synthetic graft placement cell therapy are limited their ability to restore these tissues. Biologic scaffolds composed extracellular matrix (ECM) have been prepared from numerous source tissues including small intestine, urinary bladder, liver, dermis. When properly prepared, ECM implanted into a site injury rapidly infiltrated by host cells, subjected degradation, often remodel appropriate tissue. The organs represents the secreted products resident process which provides unique microenvironmental niche that influences behavior during homeostasis injury. objective present study was develop characterize specific (M-ECM) (C-ECM), determine whether scaffold preferentially promotes restoration compared non-muscle ECM. A method decellularization developed efficiently removed cells while preserving native components, basement membrane proteins, glycosaminoglycans, growth factors. Degradation produced pepsin digestion M-ECM, C-ECM, derived intestinal submucosa (SIS-ECM) decreased proliferation promoted myogenic differentiation perivascular stem vitro. Although M-ECM more degraded than both C-ECM SIS ECM, all showed similar increase myogenesis. These studies show can be manner preserves components biologic activity. activity non-tissue vitro, myogenesis extent vivo. These results indicate is not preferred substrate remodeling SIS-ECM.