Human placental Δ5-3β hydroxysteroid dehydrogenase activity (Δ5-33 HSDH): Intracellular distribution, kinetic properties, retroinhibition and influence of membrane delipidation
作者： Françoise Ferre , Michelle Breuiller , Lise Cedard , Marie-Josèphe Duchesne , Monique Saintot
DOI: 10.1016/0039-128X(75)90050-1
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摘要: Delta5-3beta HSDH activity has been assayed either by spectrophotometric method or use of radioactive substrates. The enzymatic is equally distributed between mitochondrial and microsomal fractions verified electronic microscopy. specific comparable in both fractions, as well the optimal pH Km for NAD delta5-3beta Hut pH, sensitivity to inhibitory action various steroids are different when C19 C21 used Estrogens cyclic AMP have also an on oxidation steroids. Treatment membranes with phospholipase A releases fatty acids (mainly arachidonic) decreases activity. "Adsorbtion" bovine serum albumin partially reactivates HSDH.
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