Identification of an Operon Required for Ferrichrome Iron Utilization in Vibrio cholerae

摘要: Mutagenesis of Vibrio cholerae with TnphoA, followed by screening for fusions that were activated under low-iron conditions, led to the identification seven independent fusion strains, each which was deficient in ability utilize ferrichrome as a sole iron source growth plate bioassay and had an insertion genes encoding products homologous Escherichia coli FhuA or FhuD. Expression gene IrgB but regulated Fur. We report here map operon predicted amino acid sequence FhuA, based on nucleotide sequence. Unlike those E. fhu operon, V. utilization are located adjacent opposite orientation ATP-binding cassette transporter homolog, this gene, if disrupted, does not affect vitro.