Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles.

摘要: In the past few decades, successful theranostic application of nanomaterials in drug delivery systems has significantly improved antineoplastic potency conventional anticancer therapy. Several mechanistic advantages nanomaterials, such as enhanced permeability, retention, and low toxicity, well surface engineering with targeting moieties, can be used a tool enhancing therapeutic efficacy current approaches. Inorganic calcium phosphate nanoparticles have potential to increase antiproliferative drugs due their excellent loading efficiency, biodegradable nature controlled-release behaviour. Herein, we report novel system 5-fluorouracil (5-FU)-loaded (CaP@5-FU NPs) synthesized via reverse micelle method. The formation monodispersed, spherical, crystalline an approximate diameter 160-180 nm was confirmed by different methods. physicochemical characterization CaP@5-FU NPs done transmission electron microscopy (TEM), dynamic light scattering (DLS), field emission scanning (FE-SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD). against colorectal lung cancer cells reported. were found inhibit half population (IC50) adenocarcinoma (A549) at 32 μg/mL (HCT-15) 48.5 treatment. apoptotic induction acridine orange/ethidium bromide (AO/EB) staining examining morphological changes Hoechst rhodamine B time-dependent manner. apparent membrane bleb observed FE-SEM micrographs. up-regulated proapoptotic down-regulated antiapoptotic gene expressions further semiquantitative transcriptase polymerase chain reaction (PCR). increased intracellular reactive oxygen species (ROS) quantified flow cytometry upon NP Likewise, cell cycle analysis performed confirm induction. Our study concludes that nanocarriers system, i.e. NPs, higher compared 5-FU alone alternative antimitotic drug, which causes severe side effects when administrated alone.