Caspase-9 transduction overrides the resistance mechanism against p53-mediated apoptosis in U-87MG glioma cells.
作者： Nobusada Shinoura , Shinya Sakurai , Akio Asai , Takaaki Kirino , Hirofumi Hamada
DOI: 10.1097/00006123-200107000-00027
关键词:
摘要: Objective Conflicting reports have been published with regard to the relationship between efficacy of p53 gene therapy and status gliomas. In this study, we evaluated whether U-87MG glioma cells harboring wild-type U251 U-373MG mutated demonstrate different sensitivities p53-induced apoptosis. addition, tested transduction Bax or caspase-9, which are downstream components apoptosis, can override resistance mechanism Methods We transduced U-87MG, U251, p53, Bax, caspase-9 genes via adenovirus (Adv) vectors, induce same level respective proteins, degree Results were highly resistant Adv for (Adv-p53)-mediated whereas U-373 underwent extensive apoptosis after Adv-p53 infection. cells, elevation Fas was not as marked that observed in Endogenous expression Bcl-XL Bcl-2 greater than cells. more Bax-mediated contrast, sensitive caspase-9-mediated suggesting may p53-mediated Conclusion These results proapoptotic function induced by repressed at several steps induction circumvent mechanism.
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