Combined ion conductance and fluorescence confocal microscopy for biological cell membrane transport studies
作者： A I Shevchuk , P Novak , M A Velazquez , T P Fleming , Y E Korchev
DOI: 10.1088/2040-8978/15/9/094005
关键词:
摘要: Optical visualization of nanoscale morphological changes taking place in living biological cells during such important processes as endo- and exocytosis is challenging due to the low refractive index lipid membranes. In this paper we summarize discuss advances powerful combination two complementary live imaging techniques, ion conductance fluorescence confocal microscopy, that allows cell membrane topography be related with molecular-specific at high spatial temporal resolution. We demonstrate feasibility use microscopy image apical plasma mouse embryo trophoblast outgrowth a resolution sufficient depict single endocytic pits. This opens possibility study individual events where endocytosis plays crucial role early stages development.
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