Poly(indole-5-carboxylic acid)-functionalized ZnO nanocomposite for electrochemical DNA hybridization detection
作者： Wei Zhang
DOI: 10.1007/S10008-015-3071-9
关键词:
摘要: In this paper, a novel direct DNA electrochemical biosensor was developed for detection of breakpoint cluster region gene and the cellular abl (BCR/ABL) fusion from chronic myelogenous leukemia. Nanosized ZnO first immobilized on surface carbon paste electrode, then poly(indole-5-carboxylic acid) (PICA), new electroactive material, electropolymerized substrate by using potentiostatic method to form unique nanocomposite structure. The obtained PICA/ZnO not only served as immobilization but also reflected transduction originating hybridization without any outer indicators or complicated labeling. probe successfully conducted via covalent bonding between abundant carboxyl groups film amino at 5′-end DNA. After with target DNA, formation double-helix structure induced “signal-on” nanocomposite. dynamic range detecting complementary sequence BCR/ABL 1.0 × 10−15 10−9 mol/L impedance spectroscopy, limit estimated be 2.2 10−16 mol/L.
springer.com
sci-hub.se 参考文章(25)
J. Justin Gooding, Alison Chou, Freya J. Mearns, Elicia (Leh-See) Wong, Kellie L. Jericho, The ion gating effect: using a change in flexibility to allow label free electrochemical detection of DNA hybridisation Chemical Communications. ,vol. 2003, pp. 1938- 1939 ,(2003) , 10.1039/B305798B
Guang-Xian Zhong, Ji-Xing Ye, Feng-Qiao Bai, Fei-Huan Fu, Wei Chen, Ai-Lin Liu, Xin-Hua Lin, Yuan-Zhong Chen, Electrochemical biosensor for detection of BCR/ABL fusion gene based on isorhamnetin as hybridization indicator Sensors and Actuators B-chemical. ,vol. 204, pp. 326- 332 ,(2014) , 10.1016/J.SNB.2014.07.115
Linxia Fang, Kejing Huang, Baoling Zhang, Bing Liu, Yujie Liu, Qiuyu Zhang, Nanosheet-based 3D hierarchical ZnO structure decorated with Au nanoparticles for enhanced electrochemical detection of dopamine RSC Advances. ,vol. 4, pp. 48986- 48993 ,(2014) , 10.1039/C4RA06090C
H. Fayazfar, A. Afshar, M. Dolati, A. Dolati, DNA impedance biosensor for detection of cancer, TP53 gene mutation, based on gold nanoparticles/aligned carbon nanotubes modified electrode. Analytica Chimica Acta. ,vol. 836, pp. 34- 44 ,(2014) , 10.1016/J.ACA.2014.05.029
Jungil Park, Xueqiu You, Yunseok Jang, Yoonkyung Nam, Min Ja Kim, Nam Ki Min, James Jungho Pak, ZnO nanorod matrix based electrochemical immunosensors for sensitivity enhanced detection of Legionella pneumophila Sensors and Actuators B-chemical. ,vol. 200, pp. 173- 180 ,(2014) , 10.1016/J.SNB.2014.03.001
Yuting Wang, Shi Wang, Li Tao, Qing Min, Jin Xiang, Qiman Wang, Jianmei Xie, Yuan Yue, Shuangchan Wu, Xiaofei Li, Hong Ding, A disposable electrochemical sensor for simultaneous determination of norepinephrine and serotonin in rat cerebrospinal fluid based on MWNTs-ZnO/chitosan composites modified screen-printed electrode Biosensors and Bioelectronics. ,vol. 65, pp. 31- 38 ,(2015) , 10.1016/J.BIOS.2014.09.099
Manoj Kumar Patel, Md. Azahar Ali, Saurabh Srivastava, Ved Varun Agrawal, S.G. Ansari, Bansi D. Malhotra, Magnesium oxide grafted carbon nanotubes based impedimetric genosensor for biomedical application. Biosensors and Bioelectronics. ,vol. 50, pp. 406- 413 ,(2013) , 10.1016/J.BIOS.2013.07.006
Rafiq Ahmad, Nirmalya Tripathy, Na Keum Jang, Gilson Khang, Yoon-Bong Hahn, Fabrication of highly sensitive uric acid biosensor based on directly grown ZnO nanosheets on electrode surface Sensors and Actuators B-chemical. ,vol. 206, pp. 146- 151 ,(2015) , 10.1016/J.SNB.2014.09.026
H. Talbi, D. Billaud, G. Louarn, A. Pron, In-situ spectroscopic investigations of the redox behavior of poly(indole-5-carboxylic-acid) modified electrodes in acidic aqueous solutions. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. ,vol. 57, pp. 423- 433 ,(2001) , 10.1016/S1386-1425(00)00356-5
Ailin Liu, Kun Wang, Shaohuang Weng, Yun Lei, Liqing Lin, Wei Chen, Xinhua Lin, Yuanzhong Chen, None, Development of electrochemical DNA biosensors Trends in Analytical Chemistry. ,vol. 37, pp. 101- 111 ,(2012) , 10.1016/J.TRAC.2012.03.008