Characterization of a putative NsrR homologue in Streptomyces venezuelae reveals a new member of the Rrf2 superfamily

摘要: Members of the Rrf2 superfamily transcription factors are widespread in bacteria but their functions largely unexplored. The few that have been characterized detail sense nitric oxide (NsrR), iron limitation (RirA), cysteine availability (CymR) and sulfur (Fe-S) cluster status cell (IscR). In this study we combined ChIP- dRNA-seq with vitro biochemistry to characterize a putative NsrR homologue Streptomyces venezuelae. ChIP-seq analysis revealed rather than regulating nitrosative stress response like coelicolor NsrR, Sven6563 binds conserved motif at different, much larger set genes diverse range functions, including number regulators, required for glutamine synthesis, NADH/NAD(P)H metabolism, as well general DNA/RNA amino acid/protein turn over. Our biochemical experiments further show has [2Fe-2S] switch between oxidized reduced controls its DNA binding activity vitro. To our knowledge, both sensing domain target novel an protein, suggesting represents new member superfamily. Given redox sensitivity Fe-S tentatively named protein RsrR Redox sensitive Regulator.