Extracellular electron transfer systems fuel cellulose oxidative degradation.

摘要: Ninety percent of lignocellulose-degrading fungi contain genes encoding lytic polysaccharide monooxygenases (LPMOs). These enzymes catalyze the initial oxidative cleavage recalcitrant polysaccharides after activation by an electron donor. Understanding source electrons is fundamental to fungal physiology and will also help with exploitation LPMOs for biomass processing. Using genome data biochemical methods, we characterized compared different extracellular sources LPMOs: cellobiose dehydrogenase, phenols procured from plant or produced fungi, glucose-methanol-choline oxidoreductases that regenerate LPMO-reducing diphenols. Our demonstrate all three these transfer systems are functional their relative importance during cellulose degradation depends on lifestyle. The availability donors required activate attack polysaccharides.