Assessment of reliability of steroid radioimmunoassays

摘要: Abstract A reliable radioimmunoassay (RIA) has to have high precision and accuracy. The of the measurement plasma samples is estimated by within-assay between-assay variations. standard curve closely related sensitivity. latter can be defined as ability distinguish a dose from zero or one another. highly accurate RIA must degree specificity which achieved efficient separation steroid prior using specific antisera. checked test parallelism between increasing amounts authentic endogenous steroid. This test, however, low efficiency; it only detect considerable disturbance specificity. Nevertheless, should applied whenever new biological materials are assayed. In order attain accuracy, systematic errors (blanks, neglected influence internal standard, etc.) biases (e.g., graphical construction curve) avoided. Quality control become an indispensable part RIA.