Immediate Translation of Formin DIAPH1 mRNA after Its Exiting the Nucleus Is Required for Its Perinuclear Localization in Fibroblasts

摘要: DIAPH1 is a formin protein which promotes actin polymerization, stabilizes microtubules and consequently involved in cytoskeleton dynamics, cell migration differentiation. In contrast to the relatively well-understood signaling cascades that regulate activity, its spatial regulation of biogenesis not understood. A recent report showed synthesis confined perinuclear ER compartment through translation-dependent mRNA targeting. However, underlying mechanism local yet be elucidated. Here, we provide evidence demonstrate 5′-cap-mediated immediate translation upon exiting nucleus required for localizing compartment. This supported by data: 1) Delayed resulted loss localization mRNA; 2) Once delocalized, could retargeted region; 3) The 5′-cap dependent. These results new insights into novel synthesis. addition, these findings have led development approaches manipulating cells functional studies. Furthermore, correlation has been demonstrated using method quantify intracellular distribution protein.