Effects in live cells of a c- myc anti-gene PNA linked to a nuclear localization signal

摘要: Peptide nucleic acids (PNA) are synthetic homologs of in which the phosphate-sugar polynucleotide backbone is replaced by a flexible polyamide. In this study, PNA construct was employed as an anti-gene agent intact cells culture. The cell lines studied were derived from Burkitt's lymphomas (BL) that presented translocated and hyperexpressed c-myc oncogene. A 17-mer anti-myc PNA, complementary to unique sequence located at beginning second exon oncogene, covalently linked its N terminus nuclear localization signal (NLS) (PNA-myc(wt)-NLS). When BL exposed PNA-myc(wt)-NLS, localized predominantly nuclei rapid consequent downregulation expression occurred. Under these conditions, both completion productive cycle apoptosis inhibited.