Capillary zone electrophoresis for the quantitation of oligosaccharides formed through the action of chitinase
作者： Kyung-Bok Lee , Yeong-Shik Kim , Robert J. Linhardt
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摘要: Capillary zone electrophoresis with fluorescence detection was used to analyze the products formed by chitinase acting on N-acetylchitooligosaccharide-fluorescent conjugates. Six oligosaccharides of structure [N-acetylglucosamine(1----4)]n (where n = 1-6) were conjugated 7-amino-1,3-naphthalene disulfonic acid reductive amination. Each oligosaccharide-fluorescent conjugate purified preparative gradient polyacrylamide gel electrophoresis, semi-dry electrotransfer a positively-charged nylon membrane and recovered washing salt solution. The treating each analyzed capillary electrophoresis. treatment hexasaccharide-fluorescent also examined kinetically study action pattern this enzyme.
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