Ribonucleic acid-dependent ribonucleotide incorporation. I. Purification and properties of the enzyme.

摘要: Several enzymatic reactions are known to utilize ribonucleoside triphosphates for incorporation of ribonucleotides into ribonucleic acid polymers. Two these have been well characterized. In one, cytidine 5’-phosphate and adenosine added the nucleoside terminal end soluble RNA, forming an amino acceptor RNA terminated by trinucleotide sequence, pCpCpA (14). The other is a deoxyribonucleic acid-dependent reaction, product which with base ratios complementary primer DNA (5-S). addition, less characterized reported; in these, chiefly single monophosphate incorporated, either as addition or endonucleotide linkage (9-14). It purpose this communication report purification characterization enzyme obtained from ribonucleoprotein particles Escherichia COG that catalyzes RNAdependent ribonucleotides. reaction primarily polyriboadenylate polymer.