Molecular Cloning of Pea cDNA Encoding Cycloartenol Synthase and Its Functional Expression in Yeast

摘要: The cDNA encoding cycloartenol synthase [EC 5.4.99.8] has been isolated from pea seedling by an efficient PCR using sets of degenerate primers based on the highly conserved sequences known 2,3-oxidosqualene cyclase cDNAs. obtained contains a 2271-bp open reading frame and is predicted protein 757 amino acids with high homology (81%) to Arabidopsis thaliana synthase. PCR-amplified (ORF) inserted into pYES2, expression vector in yeast, under control galactose-inducible promoter. Significant activity found homogenate yeast transformed plasmid containing ORF.