Enhanced Dopamine Release by Dopamine Transport Inhibitors Described by a Restricted Diffusion Model and Fast-Scan Cyclic Voltammetry.

摘要: Fast-scan cyclic voltammetry (FSCV) using carbon fiber electrodes is widely used to rapidly monitor changes in dopamine (DA) levels vitro and vivo. Current analytical approaches utilize parameters such as peak oxidation current amplitude decay times estimate release uptake processes, respectively. However, are often observed with inhibitors, thereby confounding the interpretation of these parameters. To overcome this limitation, we demonstrate that a simple five-parameter, two-compartment model mathematically describes DA signals balance (r/ke) (ku), summed adsorption (kads kdes) electrode surface. Using nonlinear regression, our precisely measured obtained brain slice recordings. The extracted from curves were then validated pharmacological manipulations selectively alter vesicular or transporter (DAT)-mediated upta...