RNAi effects on actin mRNAs in Homalodisca vitripennis cells.

摘要: The xylem feeding leafhopper Homalodisaca vitripennis (H. vitripennis) is an unusually robust and efficient vector of Xylella fastidiosa, a Gram-negative bacterium which causes several very important plant diseases. Here we investigated RNA interference (RNAi) to target actin, key component insect cells whole bodies, in H. cells. RNAi effectors were delivered via lipid based transfection real-time RTPCR, hybridization, microscopic analyses employed verify effects. When actin dsRNAs used, 10-fold decrease the mRNA level was seen Altered phenotypic effects also evident transfected cells, as small interfering RNAs, hallmarks RNAi. use offers new opportunities research hemipterans, most vectors pathogens.