New insights into galactose metabolism by Schizosaccharomyces pombe: Isolation and characterization of a galactose-assimilating mutant

摘要: The fission yeast Schizosaccharomyces pombe cannot use galactose as a carbon or energy source, and little is known about metabolism in this species. Here we report isolation of galactose-assimilating mutant that grows on medium containing sole source through proofreading-deficient DNA polymerase δ variant encoded by cdc6-1. Based comparative analysis gene expression profiles the wild-type (FG2-8), found SPBPB2B2.10c (gal7+), SPBPB2B2.12c (gal10+) SPBPB2B2.13 (gal1+), homologous to Saccharomyces cerevisiae GAL7, GAL10 GAL1, respectively, SPBPB2B2.08, SPBPB2B2.09c, SPBPB2B2.11 localize close gal genes, were highly expressed dramatically induced addition galactose. gal7Δ strain, carrying an integrated ura4+ marker at gal7+ locus, grew 5-fluoroorotic acid (5-FOA)-containing medium. In contrast, FG2-8 strain could not grow 5-FOA addition, gal7+, SPBPB2B2.13, gal10+ gal1+ genes increased when carried vector, these transformants We suggest gal10+, are localized chromosomal terminal repressed silencing S. pombe. was defective making assimilation possible.