Comparison of Superdex Peptide HR 10/30 Column and TSK Gel G2000 SWXL Column for Molecular Weight Distribution Analysis of Protein Hydrolysates
作者： Guowan Su , Jiaoyan Ren , Mouming Zhao , Da-Wen Sun
DOI: 10.1007/S11947-012-0965-8
关键词:
摘要: Superdex Peptide HR 10/30 column and TSK gel G2000 SWXL were compared for molecular weight (MW) distribution analysis of pure proteins/peptides standards, pork/peanut protein hydrolysates, their peptide fractions. Results revealed that good chromatographic resolution was observed standards in the MW range 189 Da to 12,384 on column, while is more suitable standard region 6,500 75,000 Da. The test fractions by ultrafiltration or Sephadex G-25 chromatography indicated had better peptides below 3,000 than column.
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