Differential Level of Platelet Amyloid β Precursor Protein Isoforms: An Early Marker for Alzheimer Disease

摘要: Objective To determine whether a differential level of platelet amyloid β precursor protein (APP) isoforms is specifically related to Alzheimer disease (AD) and it shows correlation with the progression clinical symptoms. Design After subjects were grouped according diagnosis severity dementia, APP isoform levels in platelets compared. Setting University medical centers. Patients Thirty-two patients who fulfilled diagnostic criteria for probable AD, 25 age-matched control subjects, 16 non-AD dementia. Main Outcome Measure The evaluated by means Western blot analysis immunostaining whole platelets. Messenger RNAs transcripts also reverse transcriptase polymerase chain reaction. Results ratio between intensity 130-kd 106- 110-kd was significantly lower AD group (0.31±0.15, mean±SD) compared both controls (0.84±0.2) (0.97±0.4). Clinical Diagnostic Rating score correlated (Pearson coefficient, followed Bonferroni correction, P =.01). Reverse reaction experiments showed that all experimental groups equally expressed. Conclusions pattern altered AD. In addition, alteration positive symptoms, supporting possibility consider this peripheral parameter as marker disease. These alterations are not abnormalities messenger