Assay for nanogram quantities of DNA in cellular homogenates

摘要: Abstract The DNA concentration of a crude cellular homogenate can be measured accurately in the nanogram range using fluorescence enhancement 4′,6-diamidino-2-phenylindole (DAPI) or bisbenzimidazole (Hoechst H 33258) complexed with DNA. A simple assay has been devised including an internal standard, which allows reliable measurement and compensates for any quenching due to components buffer. is highly specific DNA; no other cell component produces significant fluorescence. response linear over broad dynamic making unknown concentrations convenient.