Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress

摘要: Methotrexate (MTX) is an antimetabolite broadly used in treatment of cancer and autoimmune diseases. MTX-induced hepatotoxicity limits its application. We investigated hepatoprotective effects turmeric liver toxicity. All experiments were performed on male Wistar albino rats that randomly divided into six groups. Group one received saline orally for 30 days (control group), groups two three extract (100, 200 mg/kg respectively) 30 days, group four single dose, MTX IP at day 30, five 100 respectively dose methoterxate (20 mg/kg) 30. Four days after injection animals sacrificed evaluated. Blood ALT AST (indicators hepatocyte injury), ALP bilirubin (markers biliary function), albumin (reflect synthetic function) as well the plasma TAS concentration (antioxidant defenses) determined. The cellular antioxidant defense activities examined tissue samples using SOD, CAT, GSH-Px oxidative stress, MDA lipid peroxidation. In addition, damage was evaluated histopathologically. significantly induced (P < 0.05) decreased capacity, while hepatoprotective. Liver microscopic evaluation showed severe centrilobular periportal degeneration, hyperemia portal vein, increased artery inflammatory cells infiltration necrosis, all histopathological changes attenuated by (200 mg/kg). Turmeric can successfully attenuate MTX-hepatotoxicity. effect partly mediated through extract’s antinflammatory activity.