Comparative study of different exposure routes on the biotransformation and genotoxicity of PAHs in the flatfish species, Scophthalmus maximus

摘要: In this study, laboratory experiments were carried out in order to come a better understanding of the fate polycyclic aromatic hydrocarbons (PAHs) marine environment and especially on their bioaccumulation, biotransformation genotoxic effects fish. Juveniles turbot (Scophthalmus maximus) exposed PAHs through different routes via (1) mixture dissolved PAHs, (2) PAH-polluted sediment (3) an oil fuel elutriate. Fish 4 days followed by 6-day depuration period. each experiment, PAH concentrations seawater tanks analysed regularly gas chromatography coupled with mass spectrometry. Muscle liver samples also for parent levels bioconcentration factors calculated. Biotransformation was evaluated measuring metabolites fish bile. Genotoxicity assessed alkaline comet assay. Regardless exposure route, muscle showed peak level 1 day after beginning exposure, decrease up background towards end except which relatively low throughout study. As consequence, no bioaccumulation observed tissues at experiment. contrast, regardless routes, rapid production biliary whole This true 1-hydroxypyrene, major metabolite pyrene. After 6 recovery clean water, significant total occurred either route displayed increase DNA strand breaks correlations between lesions erythrocytes. Overall results indicate that PAH-contaminated or dispersed elutriate leads damage The quantification bile erythrocytes appear be suitable environmental monitoring pollution case accidental spills contamination.