Inflammatory responses to micro/nano-structured titanium surfaces with silver nanoparticles in vitro

摘要: The inflammatory response to implants is a key stage of promoting angiogenesis, wound healing and regulating osteogenesis. Macrophages play an important role in inflammation due their high plasticity. However, few reports have focused on the effect antibacterial materials macrophage polarization. In this work, micro/nano-structured titanium (MNT) was obtained through micro-arc oxidization. We synthesized silver nanoparticles (AgNPs) with average diameter 20 nm immobilized AgNPs onto MNT via polydopamine generate AgNP-decorated (AgPD-MNT). Further, we investigated activity behaviors materials. X-ray photoelectron spectroscopy analysis indicated that metallic Ag dopamine/polydopamine existed AgPD-MNT surface. concentration released less than 8 ppb after 21 days. showed efficacy against Escherichia coli without cytotoxicity toward osteoblasts. proliferation morphology results had no within 5 days caused apoptosis 7 flow cytometry, enzyme-linked immuno sorbent assay quantitative real-time polymerase chain reaction manifested material groups (PD-MNT, AgPD-MNT) stimulated switching macrophages mixed phenotypes (M1–M2) early (within days), whereas expression M2 upregulated later stage. Among groups, most significant regulation Especially, induced adherent-macrophage upregulation IκB reduce inflammation. Hence, could promote risk implant-associated infection.