Antigen Retrieval in Immunohistochemistry
作者： S.-R. Shi , C.R. Taylor
DOI: 10.1016/B978-0-12-386456-7.07404-9
关键词:
摘要: The antigen retrieval (AR) technique of boiling formalin-fixed, paraffin-embedded (FFPE) tissue sections in water is a highly effective method to render immunohistochemical (IHC) staining on FFPE sections, which revolution pathology and milestone IHC. authors intend briefly summarize developments AR-IHC-based research diagnostics, with particular emphasis current challenges future directions based our data wide literature review. This article includes basic principles essential techniques AR several major issues terms standardization quantitative IHC AR, extraction nucleic acids proteins from for genomic proteomic analysis, molecular mechanism other related topics. Currently, the developing, describing plan study, we wish that combining proteomics approach AR-IHC establish sophisticated platform identifying biomarkers personalized medicine.
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