Characterization of autostimulatory and transforming growth factors from human melanoma cells.

摘要: Serum-free growth of the human malignant melanoma cell line Hs0294 is associated with production transforming factor-α and an autostimulatory mitogen (melanoma growth-stimulatory activity, MGSA). The activity characterized by stimulation anchorage-independent normal rat kidney fibroblasts competition 125I-epidermal factor for binding to cells. second MGSA, stimulates anchorage-dependent cells in serum-free culture medium. When acetic acid extracts conditioned medium are subjected Bio-Gel P-30 chromatography followed reversephase high-pressure liquid chromatography, majority elutes at 30 ± 4% acetonitrile, while major peak MGSA 35 3% acetonitrile. These data indicate that apparently dependent upon mitogen, which separable from competing produced these