Hypothermia and Rewarming Induce Gene Expression and Multiplication of Cells in Healthy Rat Prostate Tissue
作者： Helena Kaija , Lasse Pakanen , Marja-Leena Kortelainen , Katja Porvari
DOI: 10.1371/JOURNAL.PONE.0127854
关键词:
摘要: Prostate cancer has been extensively studied, but cellular stress responses in healthy prostate tissue are rarely investigated. Hypothermia is known to cause alterations mRNA and protein expressions stability. The aim of this study was use normal rat as a model order find out consequences cold exposure rewarming on the genes which either members or functionally/structurally related erythroblastic leukemia viral oncogene B (ErbB) signaling pathway. Relative amphiregulin (AMR), cyclin D1 (CyD1), cyclin-dependent kinase inhibitor 1A (p21), transmembrane form prostatic acid phosphatase (PAcP), thrombomodulin (TM) heat shock transcription factor 1 (HSF1) ventral were quantified mild (2 4.5 h at room temperature) severe +10°C) hypothermia after +10°C followed by 2 temperature 3 +28°C). AMR level, apoptotic Bcl-2 associated X B-cell CLL/lymphoma (Bax/Bcl-2) ratio proliferative index Ki-67 determined. 4.5-h hypothermia, 2-h increased expression all these genes. Elevated proliferation could be seen had its highest value longer with totally recovered body temperature. Pro-apoptotic tendency while anti-apoptosis predominant shorter only partly following promote cells possibly via ErbB
harvard.edu
paperity.org参考文章(32)
Matijasevic Z, Ludlum Db, Snyder Je, Hypothermia causes a reversible, p53-mediated cell cycle arrest in cultured fibroblasts. Oncology Research. ,vol. 10, pp. 605- 610 ,(1998)
Martin J. Carden, Ray J. Newsam, C. Mark Smales, Anne Roobol, Biochemical insights into the mechanisms central to the response of mammalian cells to cold stress and subsequent rewarming. FEBS Journal. ,vol. 276, pp. 286- 302 ,(2009) , 10.1111/J.1742-4658.2008.06781.X
F. Iacopino, G. Zelano, G. Martorana, A. Bertaccini, G. Sica, C. Giovannini, G. Lama, C. Angelucci, M. Pavone Macaluso, G. La Torre, A. D'addessi, Apoptosis-related gene expression in benign prostatic hyperplasia and prostate carcinoma. Anticancer Research. ,vol. 26, pp. 1849- 1854 ,(2006)
Marlies Van de Wouwer, Edward M. Conway, Novel functions of thrombomodulin in inflammation Critical Care Medicine. ,vol. 32, pp. S254- S261 ,(2004) , 10.1097/01.CCM.0000128036.64448.9E
Tzu-Ching Meng, Ming-Fong Lin, Tyrosine Phosphorylation of c-ErbB-2 Is Regulated by the Cellular Form of Prostatic Acid Phosphatase in Human Prostate Cancer Cells Journal of Biological Chemistry. ,vol. 273, pp. 22096- 22104 ,(1998) , 10.1074/JBC.273.34.22096
Anders Aune Tveita, Erik Sveberg Dietrichs, Torkjel Tveita, Myocardial gene expression profiling of rewarming shock in a rodent model of accidental hypothermia Cryobiology. ,vol. 64, pp. 201- 210 ,(2012) , 10.1016/J.CRYOBIOL.2012.01.009
Marlon R. Schneider, Eckhard Wolf, The epidermal growth factor receptor ligands at a glance. Journal of Cellular Physiology. ,vol. 218, pp. 460- 466 ,(2009) , 10.1002/JCP.21635
Hasan B. Alam, Sahar Hashmi, Robert A. Frankelstein, Fahad Shuja, Eugene Y. Fukudome, Yongqing Li, Baoling Liu, Marc deMoya, George C. Velmahos, Alterations in gene expression after induction of profound hypothermia for the treatment of lethal hemorrhage. Journal of Trauma-injury Infection and Critical Care. ,vol. 68, pp. 1084- 1098 ,(2010) , 10.1097/TA.0B013E3181D76BD1
C. T. Esmon, W. G. Owen, Identification of an endothelial cell cofactor for thrombin-catalyzed activation of protein C Proceedings of the National Academy of Sciences of the United States of America. ,vol. 78, pp. 2249- 2252 ,(1981) , 10.1073/PNAS.78.4.2249
Raymond C Harris, Eunkyung Chung, Robert J Coffey, EGF receptor ligands Experimental Cell Research. ,vol. 284, pp. 2- 13 ,(2003) , 10.1016/S0014-4827(02)00105-2